![]() ![]() Unlike gas lasers, its solid-state lasers have an extremely long lifetime (25,000 hrs vs 2,000 hrs), guaranteeing constant power between typical experimental sessions. The 3i Spinning Disk Confocal (SDC) is appropriate for imaging with the standard fluorophores popular in the life sciences. Alternative fluorescent nuclear stains are SYTO-Green ( Invitrogen, formerly Molecular Probes) and Draq5 ( Biostatus Ltd, or Axxora LLC, formerly Alexis). Because it lacks near-UV excitation, it is NOT appropriate for DAPI/Hoechst stains of the cell nuclei. The Ultraview Spinning Disk Confocal (SDC) is appropriate for imaging emissions at 525nm (FITC, GFP, etc), 568nm (TRITC, Texas Red, etc) and 700nm (Cy-5, YoPro, etc). On the newest machine, Meta2, the spectrometer is just as sensitive as the photomultiplier tubes, but have higher dark-noise (only relevant for dim labelling). For example, after appropriate calibration, contributions from autofluorescence can be eliminated using the Meta emission spectrometers. At each voxel/pixel of the image, a full emission spectrum ranging 411 – 753 nm, separable into 10nm-wide bins, allows extended detection range and the descrimination of overlapping emission of multiple fluorophores. In addition, both Meta confocals have an exclusive spectrometer mode ("lambda fingerprint" mode). The two Zeiss 510 Meta confocals are slightly different in 543nm/561nm laser-lines, where 561nm excitation (Meta2) is better for Texas Red fluorophore. Filters and photomultiplier tube detectors are available for the standard blue, green and red emissions of standard fluorophores. For the confocal microscopes, what laser wavelengths are available for excitation? Which fluorophores are appropriate?īoth Zeiss 510 Meta Confocals are appropriate for almost all fluorophores used in life sciences. Different cameras and detectors have different spectral sensitivities. The same considerations are operant for the low-light camera used to visualize samples. Even with the correct excitation filter, the wrong light source which lacks sufficient energy in the appropriate wavelengths won't generate usable images. Although we own multiple filter sets, there is no assurance that the filter set will work on a particular microscope. In particular, we are struggling with the database structure to record swappability/compatibility between micrscopes. , about availability for your application. Until the data are available online, feel free to ask the Facility staff, preferably via email to Wavelength information for the confocal microscopes are tabulated below.įor less common fluorophores, we are building a comprehensive database of fluorescence filter sets and corresponding microscopes. For technical reasons (for example, dearth of laser-lines for excitation), not all microscopes can visualize DAPI/Hoechst staining of cell nuclei. Such fluorophores include the standard fluorescent proteins: GFP, YFP, CFP and the standard fluorescent conjugates: Fluorescein (e.g. All of our microscopes are equipped with the basic sets to image the common fluorophores. Tashman provides window screen and screen door repair and replacement, done with our own crews (Lic # 298720) – not subcontractors and, when it comes to windows and doors, we are known for our attention to detail, so, if we do the installation, we make sure it’s done right the first time because we guarantee it.įor more information regarding replacement windows stop by Tashman Home Center (7769 Santa Monica Blvd., West Hollywood, CA 90046) or give us a call at (323) 656-1534 to set up an appointment.Even without the "optimal" filter sets, it is possible to visualize many fluorophores. To see our extensive selection of screen doors, window screens, retractable screen doors and windows, security screens, security doors and pet screens and doors, please check out the following pages: Shop our complete window screen products below and learn more about the options you have. When you shop at Tashmans you get choices without the worry of downgrading quality. We offer window screen material alternatives as well as an extensive window screen frame material and color selection. We offer custom solutions for your window screen needs that you may not have considered. We offer complete window door screens that can fit your needs and specifications. We have the widest selection of screen mesh, including UltraView, Pet Screen, alarm screen, bronze, fiberglass and metal meshes. No matter what shape or size your window or door opening, Tashman’s can custom-make the perfect window screen or screen door with frames of aluminum or wood. Windows, Doors, Retractable, Security & Pet Screens ![]()
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